yrammuS 要概79Onruthai PINYAKONG80PatiparnPunyapalakulDeveloping the granular bacteria from bacterial consortium PCY for pyrene-contaminated soil bioremediation(Project 2014)Adsorption mechanisms and interactions between pharmaceutical residues and natural organic matters on surface modified superparamagnetic adsorbents(Project 2014)65One of major environmental problem is petroleum hydrocarbon contamination resulting from activities related to petroleum industry, transportation and oil spill. This study therefore aims at developing ready-to-use bacteria capable of degrading polycyclic aromatic hydrocarbon (PAH) which is the most toxic component in petroleum oil. Bacterial consortium PCY, which has high versatility to degrade 3-4 rings PAHs, preferably pyrene, and processes pyrene dioxygenase gene (nidA) responsible for pyrene degradation, was obtained in previous study. The results in this study further revealed that the increasing of pyrene concentration resulted in the decreasing of pyrene degradation efficacy of consortium PCY. On the other hand, the presence of aliphatic compounds showed no effect on pyrene degradation. In addition, consortium PCY also exhibited its ability to degrade aliphatic compounds. The granular bacteria from strain PCY was then successfully developed using corncob as carrier material and sucrose as protective agent. A granule of bacteria mixed with agricultural material, is an alternative ready-to-use inoculum for soil bioremediation improvement owing to material’s characteristics as contaminant adsorption, increasing contaminants bioavailability, and soil porosity increment, enhancing contaminant degradation activity by aerobic bacteria. In this study, microcosm experiment demonstrated that the pyrene removal activity depends on the amount of added granular bacteria. The pyrene removal in microcosm with 15% granular bacteria was 81% which higher than those containing granular material, without consortium PCY addition. In conclusion, this inoculum could enhance pyrene removal from soil by pyrene degradation ability of consortium PCY and properties of corncob. Therefore, this ready-to-use granular bacteria is a selectness for soil bioremediation with hydrocarbon contamination.The main objective of this research is to investigate adsorption mechanism between pharmaceutical residues and various organo-functional groups grafted on surfaces of superparamagnetic porous silicas in synthetic wastewater. Adsorption kinetic of ciprofloxacin (CIP), nalidixic acid (NAL), and sulfamethoxazole (SMX) followed the pseudo-second-order kinetic model. Combination between hydrophobic interaction via van der Waals force, ¶-¶ electron-donor-acceptors interaction and electrostatic interaction was suggested to be the main CIP adsorption interaction. While the NAL adsorption mechanism was controlled by the combination between electrostatic interaction and hydrogen bonding. The hydrogen bonding was the key role in the adsorption interaction between SMX and the synthesized adsorbents. Three antibiotics adsorption did not interrupted by TA but these could be increased adsorption capacity by two phenomena; the multilayer formation between CIP and TA via electrostatic interaction, and the already adsorbed TA molecule could be interacted with the other solutes (NAL, and SMX) via ¶-¶ electron-donor-acceptors interaction. High Gradient Magnetic Separation filter (HGMS filter) was applied to separate adsorbent particle from water. The breakthrough time was increased by extending bed depth of stainless filter and decreasing flow rate and particle concentration.
元のページ ../index.html#69