84Panan RERNGSAMRAN85Chenphop SAWANGMAKE86Supeecha WITTAYALERTPANYACloning and Expression of Laccase from Agrocybe sp. CU43 in Pichia pastoris for Fluorene Degradation (Project 2015)Roles of Simvastatin on Proliferation and Osteogenic Differentiation Potential of Canine Bone Marrow-derived Mesenchymal Stem Cells (Project 2015)Effects of Chronic Kidney Disease on Intestinal Drug Transporters and CYP3A in a Mouse Model (Project 2015)63and long wavelengths when compared to that of the three-stage process absorber. It was shown that the 0.8 and 1.2 µm thick trilayer absorbers can maintain the same level of efficiency to that of the bilayer absorber of 1.8 µm thick.This is the first report of laccase genomic and cDNA gene sequences from the fungus in the genus Agrocybe. The genomic laccase sequence of Agrocybe sp. CU43 was 3,083 bp in length with putative CAAT and TATA boxes located at -161 bp and -54 bp upstream of start codon, respectively. The presence of signal peptide and signal peptidase cleavage site indicated that laccase protein was predicted to be a secreted protein. Thirteen putative introns were found intervening within the gene. cDNA sequence was cloned which was 1,563 bp in length encoding for laccase protein of 520 amino acids with predicted molecular weight about 55.9 kDa. Laccase cDNA was cloned into pPICZαA in order to express in Pichia pastoris. SDS-PAGE revealed recom-binant laccase with molecular weight higher than 70 kDa. This result indicates high glycosylation events oc-curred in the recombinant laccase. The protein will be further examined for fluorene degradation ability.This study was aimed to investigate the effects of simvastatin on proliferation and osteogenic differentiation of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs) in vitro. The results showed that the cBM-MSCs were efficiently isolated from bone marrow aspirates and could be expanded in vitro. The isolated cBM-SMCs contained an in vitro osteogenic differentiation potential as illustrated by alkaline phosphatase activity, matrix mineralization, and upregulation of osteogenic mRNA markers. Simvastatin showed no significant positive effects on cell proliferation but the significant beneficial effects were found on osteogenic induction of cBM-MSCs. Simvastatin (0.1 and 10 nM) exerted positive effects on an in vitro osteogenic differentiation of cBM-MSCs in regards of alkaline phosphatase activity, matrix mineralization, and expression of crucial osteogenic gene markers. Contrastingly, simvastatin showed negative effects on osteogenic differentiation by cDPSCs that were used as benchmark cell. Thus, the study illustrated the beneficial effects of simvastatin on osteogenic differentiation by cBM-MSCs in vitro.Chronic kidney disease (CKD) is associated with an increased bioavalability of drugs. Patients with CKD have been reported to be at risk of adverse drug reactions and drug-drug interaction since CKD may be associated with alteration of intestinal transporters and cytochrome P450 (CYP) enzyme in the intestines. The present study aimed to investigate the repercussion of CKD on gene expression of intestinal transporters (mdr1a, oatp3, mrp2), and cyp3a11 and the activity of CYP3A in the intestine. Two groups of mice were studied: CKD
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