101Hermawan JUDAWISASTRA102Ahmad FaizalBonar Tua Halomoan Marbun103Development of Biodegradable Silk Fibroin Scaffold for Tissue Engineering Applications (Project 2016)Establishment of Hairy Root Lines and Analysis of Saponin Production in the Medicinal Plant Talinum paniculatum (Javanese Ginseng) (Project 2016)Optimization of Tubing Material Selection in Production and Injection Well for Enhanced Oil Recovery and Carbon Capture Storage Application (Project 2016)70The tissue engineering field is offering a promising alternative to fulfill the limitation of autologous transplantation for damaged tissue repair. To support tissue regeneration, biocompatible, biodegradable, and high strength silk fibroin (SF) has been proposed as scaffold materials. In this research, direct dissolution in CACl2/formic acid, a faster and simpler process than traditional SF dissolution techniques, combined with salt leaching and freeze drying techniques was adapted to fabricate the scaffolds. Evaluation on the SF scaffold’s morphology, hydrophilicity, biodegradability and biocompatibility were conducted. Biodegradable SF scaffold fabrication using combination of direct dissolution and salt leaching techniques has been successfully developed. The SF scaffolds has pore size 100-500 µm with highly interconnected pores. Hydrophilicity evaluation, observation of cell attachment, MTT cytotoxicity assays and cell proliferation measurement revealed good biocompatibility properties of the scaffolds. The scaffold degraded under proteolytic enzymes. The highest degradation rate was found under incubation in Protease XIV.The continuous need for new compounds with important medicinal activities has led to the identification and characterization of various plant-derived natural products. As a part of this program, we study the production of potential pharmaceutical compound from Talinum paniculatum or widely known as Javanese ginseng. In this research, we reported the induction of transgenic hairy root lines of T. paniculatum, screening and selection for saponin production. Two Agrobacterium rhizogenes strains, ATCC and LBA9402/12 were used to induce hairy roots following infection of leaves and internodes of in vitro plants and leaf explant of ex vitro-grown plants. The effects of bacterial concentration, incubation time, and the presence of acetosyringone were also evaluated on the efficiency for hairy root induction. Both strains were able to induce hairy roots from leaf explants, but LBA9402-induced hairy roots showed faster growth rate that those from ATCC-induced strain. Saponin analysis from hairy root showed that this type of culture accumulates higher saponin content than non-transgenic root. Thus, hairy roots offer a good system to for the production of saponin in T. paniculatum.One of the main problems faced in oil and gas operations is tubing corrosion. This can lead to a decrease in oil and gas production and other problems that affect the well production economic aspects. Nowadays, there are a lot of developed technology implemented in the oil and gas industry. Technology can improve oil and gas recovery rate, in this case by using Enhanced Oil Recovery (EOR), or to utilize the well as a media to store wasted material, in this case Carbon Capture and Storage (CCS). These two technologies are carried out by conducting a fluid injection in to the wells, such as water, steam, polymer, surfactant for the
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