yrammuS 要概78Panida Thanyasrisung79Poorichaya SomparnAdaptation of Streptococcus mutans gene expressions to human breast milk(Project 2015)Quantitative Protein Expression Profiling of Anti-dsDNA antibodies in Human Mesangial cells(Project 2015)61ests in Japan. These land crabs are thought to play major roles in the matter cycle because of its mode of life; it lives in seawater during its larval stage and returns to forests after metamorphosing into small crabs. The crabs mainly consume plant material and its detritus. Therefore, they are expected to possess the ability to degrade the major components of biomass, cellulose and lignin in order to digest plant materials. In this study, we analyzed biomass-degrading activities of the land crabs and developed artificial breeding method of C. haematocheir. We found that guaiacol oxidation activity is closely related to herbivorous character of land crabs and that food is very important for metamorphosisof zoea larva.Main sugar in human breast milk is lactose which considered less cariogenicity. However, several clinical studies revealed that breastfeeding are related to the incidence of caries in preschool-children. This study aims to investigate the caries-associated genes expression of S.mutans after prolonged-cultivation in HBM. S.mutans UA159 were cultured in pooled-HBM or in brain-heart-infusion broth and then sub-cultured to the same media up to 15 passages (1 passage=11 hours). The bacterial cells were collected at the 1st, 13th and 15th passage and total RNA were extracted. Sixty-nine candidate genes related to sugar metabolism, acid production, acid tolerant and biofilm formation were selected. The gene expressions were analyzed by using Nanostring technology. Genes involved in lactose metabolism of the 1st passage of HBM-grown S. mutans increased expression more than 100 times compared to the BHI-grown cells. After exposure to HBM for 11 hours, S. mutans increase expression of genes related to sugar metabolism. Lupus nephritis (LN) is a severe organ manifestation of systemic lupus erythematosus (SLE) and a major cause of morbidity. Anti-DNA antibodies are implicated in the pathogenesis of LN and deposited in organs and elicit inflammatory damage remains unknown. In this study, we aimed to investigate the mechanism of the anti-dsDNA antibodies mediate on mesangial cells and the subsequent inflammation processes. We isolated anti-dsDNA antibodies from active LN patients by affinity chromatography. Incubation of human mesangial cells (HMC) with anti-DNA antibodies induced IL-6 and MCP-1 expression in a dose-dependent manner. We used tandem mass spectrometry to identify the protein. 780 proteins were identified and 411 proteins were down and 369 proteins were up-regulated, respectively. These protein were involved in translation regulator activity, Binding, Receptor activity, Structural molecule activity, Signal transducer activity, Catalytic activity, Transporter activity. Based on our results, we proposed that anti-dsDNA antibodies involved the inflammatory signaling pathway. This study demonstrated the protein is a novel candidate for new therapeutic target for inflammation.Rep. Grant. Res., Asahi Glass Foundation (2018)
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