yrammuS 要概92PattarasineeBhattarakosol93Nawaporn Vinayavekhin94Kasidit NootongA Development of Communication and Alert System through Eye Gaze Detection (Project 2018)Probing mechanisms of and improving 1-butanol tolerance of Bacillus subtilis by modulating levels of lipids in the membrane lipid biosynthetic pathways (Project 2018)Effects of Light Spectrums and Aeration Rates on Growth and Carotenoids Prodution in Microalgal Chlorococcum in Photobioreactor (Project 2018)69Rep. Grant. Res., Asahi Glass Foundation (2019)The human eyes are an expression of emotion explicitly when it has been stimulated from the external environ-ment. The fundamental emotional expression consists of happiness, surprise, anger, and fear. These emotions are used to identify a positive or a negative emotional state. Thus, this research aims to investigate the pattern of emotions using the external elements of eyes. The external elements compose of the interpalpebral fissure (IPF), the palpebral fissure length (PFL), and the palpebral fissure region (PFR). These elements are applied to classify the emotions between neutral and fearful. The emotional classification was developed based on the changing values of the IPF, PFL, and PFR. Moreover, an ID3 algorithm was used to classify emotions. The dataset was utilized to generate the proposed pattern by three hundred sixty images from horror-thriller-murder movies based on IMDb. This pattern was used to classify the emotions using the decision tree technique that leads to the development of an emotional classification model. The performance of the emotional classification model between the neutral emotion and the fear emotion was 95.0%.Microbes with enhanced 1-butanol tolerance have the potentials to be utilized in various biotechnological processes. To achieve the rational design of such strains, we previously conducted an untargeted metabolomics analysis of Bacillus subtilis under 1-butanol stress and uncovered a novel type of microbial responses as the alterations in the glycerolipids and phospholipids composition. However, the current knowledge about the relevance of these changes on 1-butanol tolerance remains quite limited. Here, we constructed the B. subtilis mutants with disruption in the pssA, ugtP (U), mprF (M), yfnI, and yfnI/mprF genes in the membrane lipid biosynthetic pathways. The 1-butanol tolerance test indicated markedly increased and decreased 1-butanol resistance in M and U compared to the wild-type strain under high stress level, respectively. An improved 1-butanol tolerance was in direct relation with an elevated ratio of neutral and anionic to cationic lipids in the presence of 1-butanol. Last, cell morphological studies showed the shortening of only the U cells, compared to the wild-type. All strains including U were capable of elongating by 14–24% under 1-butanol stress. Together, the studies indicated the involvement of membrane lipid biosynthetic genes on 1-butanol tolerance and al-lowed for the procurement of M with enhanced 1-butanol tolerance trait.Light quality influences biomass and carotenoid production in microalgae, yet no information is available for Chlorococcum. This work performed series of batch cultivation of Chlorococcum to assess biomass and carot-enoid production in stirred-tank and airlift photobioreactors under different light qualities and supplying strat-egies. White light was preferred for growth while blue light was suitable for carotenoid production during stationary phase. Batch cultivation in stirred-tank photobioreactors subjected to two-staged lighting yielded
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