旭硝子財団助成研究成果報告2022

82/90

Magdalena Lenny SITUMORANG112113Daryono Hadi TJAHJONO114Quy-Thinh DAO Synbiotic Modulation of Gut Microbiome, Metabolic Function and Metabolite Profiles in Pacific White Shrimp Litopenaeus vannamei Grow-out Culture in Biofloc Production System(Project 2021)Design, synthesis, and activity assay of functionalized Epidermal Growth Factor Receptor ligands as anticancer candidate for NSCLC(Project 2021)Development of a pneumatic artificial muscles based robotic orthosis for gait rehabilitation (Phase 2)(Project 2021)78旭硝子財団　助成研究成果報告（2022）The emergence of infectious diseases had resulted in heavy losses in global shrimp production, which often influenced by unstable culture water conditions. This raises the urgency to manage the water quality of shrimp, including through the application of biofloc technology. The use of synbiotic, a combination of probiotic and prebiotic, through diet supplementation also appears to be a promising strategy to improve shrimp productivity and quality. This study showed that the use of biofloc system and synbiotic supplementation provided more stable physicochemical and microbial water quality parameters, modulated the shrimp gut functional microbial activity and diversity, and improved the efficiency of feed utilization, resulting in high survival, growth and total shrimp biomass. Taken together, both biofloc system and synbiotic supplementation can be implemented as effective aquaculture technology for productivity improvement in intensive shrimp grow-out culture with minimal water exchange, towards a more sustainable global shrimp aquaculture industry.Cancer therapy has advanced to a targeted therapeutic approach using a suitable chemical molecule. Therapeutic peptide is one of pharmaceutical agents composed of a series of well-ordered amino acids. Due to its possibility to promote T-cell response, smaller and less immunogenic peptides are currently being developed. This study designed short peptides from CMYIEALDKYAC, part of the active region of epidermal growth factor loop B and studied their interaction to epidermal growth factor receptor (EGFR) via molecular dynamic simulation. Six out of eighteen peptides have retained their position inside the binding pocket during 200 ns simulation, while three peptides, CY12-63, CY12-81, and CY12-85 have shown an ability to form close-state complexes. Interaction analysis showed that all peptides interact to distinct amino acids in domain I, II, and III compared to EGF but able to remain in the binding pocket. In conclusion, CY12-63, CY12-81 and CY12-83 gave the best result and potential to be further developed as peptides targeting EGFR extracellular domain.This research aims to develop a simple mechanism but high effective robotic orthosis for gait rehabilitation. The robot is designed to support patients with movement disabilities caused by neurological pathologies such as spinal cord injury (SCI), stroke, during the training process. The robot interacts closely with humans, safety is always the top priority consideration in the design. It also has high stiffness enough to support the patient’s movement during training. In the first phase of this research, a robot orthosis was developed and tested by multi-scenarios experiments. In this phase, Unity software is used to design sample physical therapy exercises for a lower limb rehabilitation robot with the goal: doctors perform a few movements of the