yrammuS 要概106Theerayut PHENGSAART107Tanatorn KHOTAVIVATTANAPlastic separation using hybrid jig for resources recycling: effects of geometrical properties of particles on the apparent density and separation(Project 2022)Development of Novel Furocoumarin Derivatives as Anti-cancer Agents(Project 2022)77the physiological relevance of native tissues and, therefore, lead to poor predictions of compound bioactivity. Here I established a method to generate three-dimensional (3D) hepatic organoids consisting of hepatoma cells and hepatic stellate cells (HSCs) to model pathological conditions of fibrosis in culture. This model system was used to test the anti-fibrotic property of microRNA-223 (miR-223) in our recent published work. To personalize this organoid model, I devised a method to derive HSCs from human dermal fibroblasts for co-culturing with hepatocytes. Finally, I performed a screening of Thai plant extracts that prevent toxicity from lipid accumulation in hepatocytes. These compounds could be then used to test its antifibrotic potentials with the organoid model. Together, the invention of this organoid model will reveal mechanistic action of bioactive compounds in a physiological context and facilitate drug discovery from Thai medicinal plants, increasing their economic values and spurring interest in natural products as drug leads and diet supplements.The hybrid jig is a technique that combines the concepts of jig and flotation to separate mixed plastics using apparent density. However, actual waste plastics contain a wide variety of sizes and shapes that complicate separation. In this study, the effects of particle geometry (i.e., size and shape) on hybrid jig separation of crushed plastics were investigated. Plastic boards with 1-, 2-, 3-, 4-, and 5-mm thicknesses of polyethylene terephthalate (PET; specific gravity (SG) of 1.31) and polyvinyl chloride (PVC; SG of 1.38)) were crushed to obtain a size fraction of +2.0–8.0 mm. The measurement of size distribution, shape distribution, settling velocity, and attached bubble volume during water pulsation were carried out and the jig experiments both without (conventional jig) and with bubble generation (hybrid jig) were investigated. The results showed that conventional jig separation was affected by particle shape due to the unique crushing behavior of plastic boards and differences of settling velocities. In contrast, the separation via hybrid jig were similar even the particle shape of mixed plastics was different that was in line with the results of bubble attachment during water pulsation indicating that the effects of sphericity and roughness were negated.Psoralen derivatives are well known for their unique phototoxicity and also exhibits promising anti-breast cancer activity both in the presence and the absence of UVA irradiation. However, the structure-activity relationship on this scaffold remains lacking. Herein, a series of psoralen derivatives with various C-5 substituents were synthesized and evaluated for their in vitro dark and light-activated cytotoxicity against three breast cancer cell lines: MDA-MB-231, T47-D, and SK-BR-3. The type of substituents dramatically impacted the activity, with the 4-bromobenzyl amide derivative (3c) exhibiting the highest dark cytotoxicity against T47-D (IC50 = 10.14 µM), with the activity comparable to those of the reference drugs (doxorubicin, 1.46 µM; tamoxifen citrate, 20.86 µM; lapatinib 9.78 µM). On the other hand, the furanylamide 3g exhibits the highest phototoxicity against SK-BR-3 cells with the IC50 of 2.71 µM, which is almost 10-fold increase compared to the parent compound, methoxsalen. Moreover, these derivatives showed exceptional selectivity towards HER2+ (SK-BR-3) over the HER2− (MDA-MB-231) breast cancer cell lines, which correlates well with the results from the molecular docking study, revealing that 3g formed favorable interactions within the active site of the HER2. Additionally, the cell morphology of SK-BR-3 cells suggested that the significant phototoxicity was related to induction of cell apoptosis. Most of the synthesized psoralen derivatives possess acceptable physicochemical properties and are suitable for being further developed as a novel anti-breast cancer agent in the future.Rep. Grant. Res., Asahi Glass Foundation (2023)
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