101Supansa YODMUANG102Phatsawee JANSOOK103Supawadee UMTHONGDevelopment of Imitation Shark Fins by Tissue Engineering Technology(Project 2023)Development of Imatinib / Cyclodextrin Complex-Loaded Niosomes for Targeted Lung Cancer Therapy(Project 2023)Characterization of human and bat MARCH2 proteins in restriction of viral envelope glycoproteins(Project 2023)Shark fins are a popular delicacy in Asia, symbolizing health and wealth. However, the harvesting process is unsustainable, and ocean pollution has led to the accumulation of heavy metals in sharks, posing health risks. Using tissue engineering technology, we successfully created imitation shark fins by combining porcine chondrocytes with an edible hydrogel made from alginate, gelatin, and xanthan gum. We found that the percentage of xanthan gum influenced the hydrogel's properties. The hydrogel containing 2% (w/v) xanthan gum exhibited excellent viscosity and swelling, supporting tissue formation. The cell/hydrogel mixture was able to form a brush-like structure using a custom-made 3D3D bioprinter. Chondrocytes showed high viability in the hydrogel after four weeks of culture. Our research aims to leverage the existing pork industry, which already provides a wide range of meet products. We demonstrated that transforming pig cells and tissues into imitation shark fins is a challenging goal achievable through tissue engineering and 3D bioprinting. Lung cancer is a leading cause of cancer-related mortality worldwide. Effective cancer treatment aims to deliver drugs that specifically target cancer cells at the optimal dose. Imatinib (IMB), a selective kinase inhibitor, shows therapeutic efficacy against lung cancer, but its low solubility in water limits its use. This study investigated the formation of IMB/cyclodextrin (CD) inclusion complexes and the preparation of IMB-loaded niosomes, with and without TPGS, using the thin-film hydration method. These formulations were analyzed for particle size, size distribution, zeta potential, and entrapment efficiency (%EE). IMB demonstrated a high affinity for γCD and HPγCD, as evidenced by phase-solubility studies, solid- and solution-state characterizations, and molecular docking. The prepared niosomes were nanosized and spherical with high %EE. IMB-loaded niosomes containing TPGS showed significantly increased cytotoxic activity against the A549 lung cancer cell line at a concentration of 5 μM. These results indicate that IMB-loaded niosomes could be a promising nanocarrier for cancer therapy.Hosts and pathogens develop ways to coexist, with hosts using defense proteins like Membrane-Associated RING-CH (MARCH) proteins. MARCH2, found in humans and fruit bats (Pteropus), shows antiviral activity against viruses like Human immune deficiency virus type 1 (HIV-1), Influenza A virus (IAV), Ebolavirus (EBOV), and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).). Unlike humans, bats don't get sick from these infections. This study explores the antiviral functions of human and bat MARCH2, focusing on an eight-amino acid addition in bat MARCH2. We created and tested different MARCH2 plasmids and found that this addition boosts anti-HIV-1 activity. These results provide a foundation for further testing on Nipah, Ebola, and Coronaviruses. Our findings emphasize the importance of studying bat-specific MARCH2 sequences and their role in antiviral defense. Future research will aim to use these mechanisms for new antiviral treatments, leading to innovative strategies against various viral infections. 76
元のページ ../index.html#80